Development of time-resolved fluoroimmunoassay kit for detection of IgM antibodies against hepatitis B core antigen / 南方医科大学学报
Journal of Southern Medical University
;
(12): 84-86, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-339059
ABSTRACT
<p><b>OBJECTIVE</b>To prepare a time-resolved fluoroimmunoassay (TRFIA) kit for clinical detection of IgM antibodies to hepatitis B core antigen (HBc).</p><p><b>METHODS</b>Immunocapture method was used to develop the TRFIA kit for detection of the anti-HBc IgM antibodies, and the precision, cross-reactivity and sensitivity of the kit were tested with the clinical serum samples.</p><p><b>RESULTS</b>The intra- and inter-assay coefficients of variation of the TRFIA kit were 4.8%-7.2% and 7.5%-8.6%, respectively, and no cross-reactivity with anti-HBs, anti-HBc-IgG or anti-HBe was found. Comparison of the results of the TRFIA kit and enzyme-linked immunosorbent assay (ELISA) demonstrated greater sensitivity of the kit than ELISA in detecting the anti-HBc IgM antibodies in 584 serum samples. According to the detection results in 300 serum samples from healthy donors, the cutoff value of the TRFIA kit was 4.5 times of the fluorescence value of the negative control.</p><p><b>CONCLUSION</b>This TRFIA kit for detecting anti-HBc IgM antibodies meets the demand for clinical application and can replace the ELISA kits.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Juego de Reactivos para Diagnóstico
/
Virología
/
Sangre
/
Inmunoglobulina M
/
Fluoroinmunoensayo
/
Sensibilidad y Especificidad
/
Alergia e Inmunología
/
Hepatitis B
/
Anticuerpos contra la Hepatitis B
/
Antígenos del Núcleo de la Hepatitis B
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2009
Tipo del documento:
Artículo
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