Inhibition of survivin expression in liver cancer cells by shRNA / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 712-715, 2003.
Article
en Zh
| WPRIM
| ID: wpr-339104
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the plasmid containing short hairpin RNA (shRNA) of survivin in order to suppress the expression of survivin gene in HepG2 and SMMC-7721.</p><p><b>METHODS</b>Two 20 to 21 bp reverse repeated motifs of survivin target sequence with 4 bp or 8 bp spacer were synthesized respectively and inserted into plasmid pTZU6+1 to generate the plasmid pshRNA-survivin1 and pshRNA-survivin2; plasmid pEGFP-C1-survivin and pshRNA-survivin1 or pshRNA-survivin2 plasmid were cotransfected into liver cancer cell HepG2 and SMMC-7721 to detect effect of GFP expression respectively and analyze the inhibition of survivin gene.</p><p><b>RESULTS</b>The recombinant plasmid pshRNA-survivin1 and pshRNA-survivin2 were successfully constructed. The recombinant plasmids suppress the survivin expression by 80% in HepG2 and SMMC-7721.</p><p><b>CONCLUSION</b>The result showed that the short hairpin RNA of survivin can efficiently suppress it's expression in HepG2 and SMMC-7721.</p>
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Índice:
WPRIM
Asunto principal:
Terapéutica
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Terapia Genética
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Regulación Neoplásica de la Expresión Génica
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ARN Interferente Pequeño
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Interferencia de ARN
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Proteínas Inhibidoras de la Apoptosis
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Genética
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Neoplasias Hepáticas
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Proteínas Asociadas a Microtúbulos
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Proteínas de Neoplasias
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Año:
2003
Tipo del documento:
Article