Expression and significance of the matrix metalloproteinase inhibitor RECK gene in prostate cell strains / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expression and significance of RECK gene and MMP-9 in prostate cell strains such as BPH-1, DU45, LNCaP and PC-3.</p><p><b>METHODS</b>The expression of mRNA of RECK and MMP-9 was measured by RT-PCR and RECK protein was evaluated by Western blot.</p><p><b>RESULTS</b>The mRNA level of the RECK gene in the prostate carcinoma cell strains, such as DU45, LNCaP and PC-3, was lower than that in the benign prostate hyperplasia cell line BPH-1, while MMP-9 had a higher expression. The protein level of RECK in DU-45, LNCaP and PC-3 was lower than that in the BPH-1.</p><p><b>CONCLUSION</b>The RECK gene is supposed to be a kind of tumor suppressor gene, which may act by inhibiting the activity of MMP-9.</p>