Genome organization of the SARS-CoV / 基因组蛋白质组与生物信息学报·英文版
Genomics, Proteomics & Bioinformatics
;
(4): 226-235, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-339503
ABSTRACT
Annotation of the genome sequence of the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) is indispensable to understand its evolution and pathogenesis. We have performed a full annotation of the SARS-CoV genome sequences by using annotation programs publicly available or developed by ourselves. Totally, 21 open reading frames (ORFs) of genes or putative uncharacterized proteins (PUPs) were predicted. Seven PUPs had not been reported previously, and two of them were predicted to contain transmembrane regions. Eight ORFs partially overlapped with or embedded into those of known genes, revealing that the SARS-CoV genome is a small and compact one with overlapped coding regions. The most striking discovery is that an ORF locates on the minus strand. We have also annotated non-coding regions and identified the transcription regulating sequences (TRS) in the intergenic regions. The analysis of TRS supports the minus strand extending transcription mechanism of coronavirus. The SNP analysis of different isolates reveals that mutations of the sequences do not affect the prediction results of ORFs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Transcripción Genética
/
Composición de Base
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Sistemas de Lectura Abierta
/
Genoma Viral
/
Análisis de Secuencia
/
Biología Computacional
/
Sustitución de Aminoácidos
/
Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Genomics, Proteomics & Bioinformatics
Año:
2003
Tipo del documento:
Artículo
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