The C-terminal portion of the nucleocapsid protein demonstrates SARS-CoV antigenicity / 基因组蛋白质组与生物信息学报·英文版
Genomics, Proteomics & Bioinformatics
;
(4): 193-197, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-339507
ABSTRACT
In order to develop clinical diagnostic tools for rapid detection of the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) and to identify candidate proteins for vaccine development, the C-terminal portion of the nucleocapsid (NC) gene was amplified using RT-PCR from the SARS-CoV genome, cloned into a yeast expression vector (pEGH), and expressed as a glutathione S-transferase (GST) and Hisx6 double-tagged fusion protein under the control of an inducible promoter. Western analysis on the purified protein confirmed the expression and purification of the NC fusion proteins from yeast. To determine its antigenicity, the fusion protein was challenged with serum samples from SARS patients and normal controls. The NC fusion protein demonstrated high antigenicity with high specificity, and therefore, it should have great potential in designing clinical diagnostic tools and provide useful information for vaccine development.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Levaduras
/
Proteínas Recombinantes de Fusión
/
Ensayo de Inmunoadsorción Enzimática
/
Genoma Viral
/
Clonación Molecular
/
Proteínas de la Nucleocápside
/
Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
/
Alergia e Inmunología
/
Vectores Genéticos
/
Genética
Límite:
Humanos
Idioma:
Inglés
Revista:
Genomics, Proteomics & Bioinformatics
Año:
2003
Tipo del documento:
Artículo
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