The effects of cell growth factors on proliferation and migration of coronary artery endothelial cells / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To study the effects of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) on proliferation and migration of bovine coronary artery endothelial cells (BCAEC) in vitro.</p><p><b>METHODS</b>BCAECs were isolated and cultured in vitro, and divided into control group, VEGF group and HGF group. BCACEs proliferation were measured using MTT, and their migration was observed using reverse microscope.</p><p><b>RESULTS</b>The OD value of control, VEGF and HGF group were 0.22 +/- 0.01, 0.40 +/- 0.14, 0.44 +/- 0.15 respectively. The proliferation ratio of BCAECs in VEGF and HGF group was 81.8% +/- 16.9%, 100.0% +/- 21.1% respectively. There was no migration in control group, but significant migration in VEGF and HGF group.</p><p><b>CONCLUSION</b>Both VEGF and HGF can promote proliferation and migration of BCAECs, the effect of HGF is stronger than VEGF.</p>