Establishment of the model of oxygen-glucose deprivation in vitro rat hippocampal neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 197-200, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-339641
ABSTRACT
<p><b>AIM</b>To establish the model of oxygen-glucose deprivation in vitro rat hippocampal neurons.</p><p><b>METHODS</b>The hippocampal neurons cultured for 12 d were exposed to combined oxygen-glucose deprivation for 0.5-4 h and then cultured with original medium in normoxia for 24 h. Auto-biochemical analyzer determined LDH activity. The change of neuronal morphology and neuron survival were observed by converted contrast microscope and assessed by photography analysis system. Neuron apoptosis was detected by using the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nickel end labeling (TUNEL) method.</p><p><b>RESULTS</b>The neurons swelled, LDH release increased and neuron survival decreased after gradually oxygen-glucose deprivation. The percentage of apoptosis increased obviously 24 h after recovering the supply of oxygen and glucose.</p><p><b>CONCLUSION</b>The model of oxygen-glucose deprivation in vitro rat hippocampal neurons is established successfully by using the modified ACSF (artificial cerebral spinal fluid) with serum and glucose free.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxígeno
/
Fisiología
/
Hipoxia de la Célula
/
Células Cultivadas
/
Ratas Wistar
/
Biología Celular
/
Glucosa
/
Hipocampo
/
Animales Recién Nacidos
/
Neuronas
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2003
Tipo del documento:
Artículo
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