The effect and mechanism of Salvia miltiorrhiza monomer IH764-3 on proliferation and collagen synthesis of hepatic stellate cells stimulated by H2O2 / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 78-81, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-339675
ABSTRACT
<p><b>AIM</b>To investigate the inhibiting role of Salvia miltiorrhiza monomer IH764-3 in proliferation and collagen synthesis and the effect on the expression of focal adhesion kinase in hepatic stellate cells (HSCs) stimulated by H2O2 in vitro, so as to provide evidences for preventing hepatic fibrosis.</p><p><b>METHODS</b>The effects of IH764-3 on the proliferation and collagen synthesis of HSCs were examined by 3H-TdR and 3H-proline incorporation assay, respectively. The expression of FAK mRNA was examined by RT-PCR in lysates of cultured HSCs.</p><p><b>RESULTS</b>The proliferation and collagen synthesis were inhibited in dose-dependent and time-dependent manners. The expressions of FAK mRNA in the IH764-3 (10 microg/ml, 20 microg/ml, 30 microg/ml, 40 microg/ml) groups were significantly lower than that in the control group.</p><p><b>CONCLUSION</b>The present results demonstrate that IH764-3 can inhibit HSC proliferation and collagen synthesis. Meanwhile the mRNA transcription of FAK is reduced significantly, FAK as a signal molecule, can be involved in the effect of IH764-3. This may be one of the anti-fibrotic mechanisms of Salvia Miltiorrhiza.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Medicamentos Herbarios Chinos
/
Transducción de Señal
/
Células Cultivadas
/
Colágeno
/
Salvia miltiorrhiza
/
Biología Celular
/
Proliferación Celular
/
Quinasa 1 de Adhesión Focal
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2003
Tipo del documento:
Artículo
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