Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 57-59, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-339680
ABSTRACT
<p><b>AIM</b>To investigate the influence and mechanisms of 17beta-estradiol on the CTP phosphorylcholine cytidylyltransferase (CCT) activity from cultured lung explants without serum.</p><p><b>METHODS</b>We detected the amount of [M-14C] choline incorporation into phosphatidylcholine so as to reflect CCT activity by liquid scintillation.</p><p><b>RESULTS</b>(1) 17beta-estradiol increased the CCT activity in dose-dependence and time-dependence. (2) Both the protein kinase C inhibitor H-7 and calmodulin antagonist W-7 abolished the stimulatory effect of 17beta-estradiol (3 x 10(-6) mol/L) on the CCT activity.</p><p><b>CONCLUSION</b>17beta-estradiol can increase CCT activity in cultured lung explants, its mechanism is related to protein kinase C and calmodulin.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Técnicas In Vitro
/
Proteína Quinasa C
/
Calmodulina
/
Medio de Cultivo Libre de Suero
/
Ratas Wistar
/
Citidililtransferasa de Colina-Fosfato
/
Estradiol
/
Pulmón
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2003
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS