Effect of hypoxia on expression of iNOS mRNA in cultured rat astrocytes / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 12-15, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-339692
ABSTRACT
<p><b>AIM</b>To explore the effects of hypoxia on expression of inducible nitric oxide synthase (iNOS) mRNA in cultured rat astrocytes.</p><p><b>METHODS</b>Cultured rat astrocytes were randomly divided into 4 groups glutamate group (G), hypoxic group (H), hypoxia + glutamate group (H + G) and the control (C). Cells of control group were exposed to normoxic (95% air, 5% CO2) condition, and cells of G and H + G were incubated with 100 micromol/L L-glutamate, cells of H and H + G exposed to hypoxic conditions (5% CO2, 95% N2) at 37 degrees C. Each group had five timepoints which included 0 h, 3 h, 6 h, 12 h, 24 h, respectively. Expression of mRNAs of iNOS were detected with reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Expression of iNOS mRNA was not detectable in G and C, while it increased dramatically and continuously from 6 h to 24 h in H and G + H. Expression of iNOS mRNA was significantly higher in H than both in G and C at 6 h, 12 h and 24 h, and expression of iNOS mRNA was the highest of all groups in G + H.</p><p><b>CONCLUSION</b>Hypoxia upregulates the expression of iNOS mRNA in cultured astrocytes. Glutamate does not induce the expression of iNOS mRNA but enhance the effect of hypoxia, which is maybe one of the adaptive mechanisms of hypoxia-induced cerebral dilation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
ARN Mensajero
/
Hipoxia de la Célula
/
Células Cultivadas
/
Corteza Cerebral
/
Astrocitos
/
Ácido Glutámico
/
Biología Celular
/
Óxido Nítrico Sintasa de Tipo II
/
Genética
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2003
Tipo del documento:
Artículo
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