Inhibitory effects of kappa-opioid receptor stimulation on cultured myocardial cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 358-361, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-339714
ABSTRACT
<p><b>AIM</b>In order to study the effects of kappa-opioid receptor activation, we used cultured cardiomyocytes to study the inhibitory effects of U50,488H on cellular proliferation, protein content in the presence or absence of nor-binaltorphimine (nor-BNI).</p><p><b>METHODS</b>The cellular proliferation was determined with crystal violet staining and the protein content was assayed with Lowry's method.</p><p><b>RESULTS</b>A kappa-opioid receptor agonist U50,488H at 0.1 micromol/L-10 micromol/L inhibited the cellular proliferation and protein content of cultured myocardial cells in a dose-dependent manner. The inhibitory effects of U50,488H were completely blocked by pretreatment with nor-BNI, a specific kappa-opioid receptor antagonist at 1 micromol/L.</p><p><b>CONCLUSION</b>U50,488H inhibited the cultured myocardial cells' growth. The inhibitory effects of U50,488H are involved in mediating the action of kappa-opioid receptor stimulation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Células Cultivadas
/
Ratas Sprague-Dawley
/
Receptores Opioides kappa
/
3,4-Dicloro-N-metil-N-(2-(1-pirrolidinil)-ciclohexil)-bencenacetamida, (trans)-Isómero
/
Miocitos Cardíacos
/
Procesos de Crecimiento Celular
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2002
Tipo del documento:
Artículo
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