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Analysis of full-length gene sequence of rabies vaccine virus aG strain / 病毒学报
Chinese Journal of Virology ; (6): 404-409, 2013.
Artículo en Chino | WPRIM | ID: wpr-339937
ABSTRACT
To sequence and analyze the full-length gene sequence of rabies vaccine virus aG strain. The full-length gene sequence of aG strain was amplified by RT-PCR by 8 fragments,each PCR product was cloned into vector pGEM-T respectively, sequenced and assemblied; The 5' leader sequence was sequenced with method of 5' RACE. The homology between aG and other rabies vaccine virus was analyzed by using DNAstar and Mega4. 0 software. aG strain was 11 925nt(GenBank accession number JN234411) in length and belonged to the genotype I . The Bioinformatics revealed that the homology showed disparation form different rabies vaccine virus. the full-length gene sequence of rabies vaccine virus aG strain provided a support for perfecting the standard for quality control of virus strains for production of rabies vaccine for human use in China.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Filogenia / Rabia / Virus de la Rabia / Especificidad de la Especie / Virología / Datos de Secuencia Molecular / Vacunas Antirrábicas / Secuencia de Bases / China / Secuencia de Aminoácidos Límite: Humanos País/Región como asunto: Asia Idioma: Chino Revista: Chinese Journal of Virology Año: 2013 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Filogenia / Rabia / Virus de la Rabia / Especificidad de la Especie / Virología / Datos de Secuencia Molecular / Vacunas Antirrábicas / Secuencia de Bases / China / Secuencia de Aminoácidos Límite: Humanos País/Región como asunto: Asia Idioma: Chino Revista: Chinese Journal of Virology Año: 2013 Tipo del documento: Artículo