The establishment of a new resequencing pathogen microarray-based assay for detecting unexplained respiratory tract infections / 病毒学报
Chinese Journal of Virology
;
(6): 137-142, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-339962
ABSTRACT
Resequencing Pathogen Microarray (RPM) is a new pathogen detection and identification technology based on DNA microarray. In order to apply RPM in the detection of unexplained infection and as a result, to improve the emergency response capacity, a new RPM-based respiratory pathogens detection assay was developed to simultaneously detect 19 common respiratory viruses, 9 influenza A viruses (Flu A),11 human rhinoviruses(HRV), 28 enteroviruses and 18 rare respiratory viruses. The specificity of multiplex system was examined by confirmed positive specimens for 16 common respiratory virus. The sensi-tivity was evaluated by serial ten-fold dilutions of plasmids or in vitro-transcribed RNA. RPM could detect and differentiate 16 virus types/subtypes at 10 - 1 000 copies/reaction level. Nucleic acids of 8 throat swabs with unexplained respiratory tract infections were pooled and detected by the new assay. The RPM result was verified by common PCR followed by sequencing as well as PLEX-ID (Abbott). Except for a false-positive of PIV1, no difference among the three assays was found. These results indicate the assay based on the new RPM is a highly sensitive, high throughput test for the detection of respiratory virus infections, which is significant for the management of emergent and epidemic infectious disease.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Infecciones del Sistema Respiratorio
/
Virología
/
Virus
/
Sensibilidad y Especificidad
/
Clasificación
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Diagnóstico
/
Genética
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Límite:
Niño
/
Child, preschool
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2013
Tipo del documento:
Artículo
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