Establishment and identification of A new HPV positive esophageal cancer cell line / 病毒学报
Chinese Journal of Virology
;
(6): 119-125, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-339965
ABSTRACT
In order to explore the relationship between human papilloma virus ( HPV) and upper gastrointestinal cancer(esophageal cancer), An esophageal squamous cell carcinoma(ESCC) tissue was obtained from a 76 year old Chinese female patient from Anyang city, a high-incidence area for esophageal cancer, in China. Transplanted tumor was formed through direct SCID mouse tumorigenicity experiment and cultured monolayer cells were obtained after several passages and screenings Immunofluorescence test, cell growth curve, soft agar assay, chromosome analysis and tissues HE staining were also performed to confirm the epithelial cell origin. Cell DNA STR typing results showed that no three alleles was observed,indicating no contamination of human cells. DNA analysis revealed the presence of HPV type 18 DNA in this cell line. DOLINK test found the E6 protein expression of HPV virus. We concluded that the established cell line is a new esophageal squamous cell-origincarcinoma cell line with HPV DNA positive and expression of viral oncoprotein. It provides new cytologic material for performing etiology studies on the occurrence and development of esophageal cancer.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Infecciones Tumorales por Virus
/
Virología
/
Neoplasias Esofágicas
/
Células Tumorales Cultivadas
/
Carcinoma de Células Escamosas
/
China
/
Ratones SCID
/
Infecciones por Papillomavirus
/
Biología Celular
/
Proliferación Celular
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2013
Tipo del documento:
Artículo
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