Effect of RelB on HIV-1 Vpr-mediated transcription activation and cell G2/M arrest / 病毒学报
Chinese Journal of Virology
;
(6): 44-50, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-339976
ABSTRACT
Vpr, an auxiliary protein of HIV-1(Human immunodeficiency virus type 1), exerts important functions to promote viral replication and AIDS progression. In this study, we performed a yeast two-hybrid screening assay using human cDNA library to further investigate the molecular mechanism of various functions of Vpr RelB, a key protein in NF-kappaB signaling pathway, was identified as a Vpr interaction protein by co-immunoprecipitation. Further investigations indicated that RelB not only promoted the Vpr-mediated activation of NF-kappaB reporter gene, but also enhanced the transactivation of HIV LTR. Moreover, the results showed that RelB promoted Vpr-induced cell cycle G2/M arrest. Collectively, these results indicated that RelB might interact with Vpr and regulate its transcriptional activation and cell cycle arrest.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Células HeLa
/
Activación Transcripcional
/
División Celular
/
Fase G2
/
Duplicado del Terminal Largo de VIH
/
FN-kappa B
/
Factor de Transcripción ReIB
/
Productos del Gen vpr del Virus de la Inmunodeficiencia Humana
/
Puntos de Control del Ciclo Celular
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2013
Tipo del documento:
Artículo
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