Development of real-time fluorescent quantitative PCR method for detecting Bombyx mori cytoplasmic polyhedrosis virus / 病毒学报
Chinese Journal of Virology
;
(6): 12-16, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-339981
ABSTRACT
A pair of specific primers were designed according to the published 118 bp conserved sequence of polyhedrin gene of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) in GenBank and a serial dilutions of a recombinant plasmid were prepared and used to generate standard curves, to establish a real-time fluorescent quantitative PCR method for detection of BmCPV. The results showed that the linear relationship between virus concentration (X) and Ct (Y) was Y = -3. 582 lgX + 38.748 with the correlation coefficient R2 = 0999. The method was very sensitive, specific and reproducible. It can be applied in the rapid detection of BmCPV and the prevalence investigation of this disease.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Reoviridae
/
Bombyx
/
Virología
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Fluorescencia
/
Genética
/
Métodos
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2013
Tipo del documento:
Artículo
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