Cloning and functional research of Arp2/3-P40/ARPC1 subunit of Sf9 cells / 病毒学报
Chinese Journal of Virology
;
(6): 601-608, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-339998
ABSTRACT
The baculovirus-induced actin polymerization is mainly associated with the virus nucleocapsid protein P78/83, which is homologous with WASP proteins that can activate Arp2/3 complex and induce the actin polymerization. In order to explore the role of Arp2/3 complex in the baculovirus replication, the P40 subunit of Arp2/3 complex from Sf9 (Spodoptera frugiperda 9) cell line was cloned and characterized. Immunofluorescent microscopy assay indicated that P40 was recruited to the inner-side of nuclear membrane during virus infection, which was in accordance with nuclear F-actin distribution in virus-infected cells as documented in our previous research, suggesting P40 could be used to track Arp2/3 complex subcellular distribution changes during virus infection. In addition, co-immunoprecipitation assay demonstrated that P40 interacted with P78/83 only in virus-infected cells, suggesting that actin polymerization induced by P78/83-Arp2/3 complex during baculovirus infection was regulated by some unidentified virus factors.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Unión Proteica
/
Virología
/
Datos de Secuencia Molecular
/
Línea Celular
/
Química
/
Alineación de Secuencia
/
Secuencia de Aminoácidos
/
Clonación Molecular
/
Nucleopoliedrovirus
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2012
Tipo del documento:
Artículo
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