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Effects of fluoride on proliferation and differentiation of rat osteoblasts in vitro and the antagonistic action of vitamin C / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 250-252, 2003.
Artículo en Chino | WPRIM | ID: wpr-340029
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of fluoride on the proliferation and differentiation of osteoblasts in sucking rats and the antagonism of vitamin Cin vitro.</p><p><b>METHODS</b>The enzyme digesting method was used to isolate the rat osteoblasts; the proliferative response was determined by the percents of reduced alamarBlue; the activity of alkaline phosphatase (ALP) was measured by ELISA method.</p><p><b>RESULTS</b>The proliferation of sucking rat osteoblasts was increased at 0.10 - 1.00 mmol/L of NaF, whereas inhibited at >or= 2.00 mmol/L. ALP activity was increased at 0.01 - 0.05 mmol/L of NaF, and decreased at >or= 0.10 mmol/L. The inhibition on proliferation and differentiation at 2 mmol/L NaF was antagonized by vitamin C.</p><p><b>CONCLUSION</b>Fluoride had a two-phase effect on osteoblasts, vitamin C could antagonize the inhibitory effect of higher concentration of fluoride on proliferation and differentiation of osteoblasts.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteoblastos / Farmacología / Ácido Ascórbico / Ensayo de Inmunoadsorción Enzimática / Diferenciación Celular / Ratas Wistar / Biología Celular / Proliferación Celular / Fosfatasa Alcalina / Fluoruros Límite: Animales Idioma: Chino Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteoblastos / Farmacología / Ácido Ascórbico / Ensayo de Inmunoadsorción Enzimática / Diferenciación Celular / Ratas Wistar / Biología Celular / Proliferación Celular / Fosfatasa Alcalina / Fluoruros Límite: Animales Idioma: Chino Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Año: 2003 Tipo del documento: Artículo