Exposure to power-frequency magnetic fields can induce activation of P38 mitogen-activated protein kinase / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 252-255, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-340090
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of 50 Hz power-frequency magnetic fields on signal transduction pathway of P38 mitogen-activated protein kinase (P38 MAPK), and explore the cellular signal transduction mechanism of the biological effects induced by power-frequency magnetic fields.</p><p><b>METHODS</b>Chinese hamster lung (CHL) cell line was exposed to power-frequency magnetic fields with two intensities(0.1 and 0.4 mT) for different exposure durations. The cytoplasmic protein was extracted. The phosphorylated(activated) and non-phosphorylated P38 MAPK and MKK3/MKK6 were measured by Western blotting analysis with their specific corresponding antibodies.</p><p><b>RESULTS</b>Power-frequency magnetic fields at 0.4 mT for 10 min could transitorily induce the activation of P38 MAPK and after 15 min the phosphorylation of P38 MAPK restored to control level, while 0.1 mT power-frequency magnetic fields could not induce the activation of P38 MAPK within 24 h. However, both 0.1 mT and 0.4 mT power-frequency magnetic fields could not phosphorylate(activate) the MKK3/MKK6, which is a general upstream kinase of P38 MAPK.</p><p><b>CONCLUSION</b>Power-frequency magnetic fields could transitorily activate the P38 MAPK, but not MKK3/MKK6. The activation mechanism of P38 MAPK needs to be further identified.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Efectos de la Radiación
/
Línea Celular
/
Cricetulus
/
MAP Quinasa Quinasa 3
/
MAP Quinasa Quinasa 6
/
Proteínas Quinasas p38 Activadas por Mitógenos
/
Activación Enzimática
/
Pulmón
/
Magnetismo
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Año:
2002
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS