Effects of Yikunning on the apoptotic rate and expression of caspase-3 in rat ovaries during perimenopausal period / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 318-321, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-340162
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Yikunning (compound of Chinese traditional Medicine, YKN) on the apoptotic rate and expression of caspase-3 in rat ovaries during perimenopausal period.</p><p><b>METHODS</b>Thirty female Wistar rats during perimenopausal period were selected by unforced aging. Then the rats were divided into 3 groups randomly YKN group, livial control group and aged control group. Ten young female rats were selected as young control group. Intragastric administrations were conducted for 4 weeks once daily continuously. The apoptotic rate in rat ovaries were detected by TUNEL. The expression of caspase-3 mRNA and protein in rat ovaries were detected by RT-PCR and Western blot, respectively.</p><p><b>RESULTS</b>The apoptotic rate in rat ovaries in YKN group was lower than that in aged control group, which showed difference between them (P < 0.01). The levels of caspase-3 mRNA and protein in rat ovaries in YKN group were lower than those in aged control group, which showed differences among them (P < 0.01).</p><p><b>CONCLUSION</b>YKN can decrease the apoptotic rate and down-regulate the expression of caspase-3 mRNA and protein in rat ovaries of during perimenopausal period. It may be one of the molecular mechanisms of YKN postponed the ovarian failure and cured perimenopausal syndrome.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ovario
/
Farmacología
/
Medicamentos Herbarios Chinos
/
Ratas Wistar
/
Apoptosis
/
Biología Celular
/
Perimenopausia
/
Caspasa 3
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2010
Tipo del documento:
Artículo
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