Establishing the model of kappaB-decoy inhibiting the activity of NF-kappaB in PC12 cells / 中国应用生理学杂志

ABSTRACT

<p><b>OBJECTIVE</b>Observing the time course and establishing the model of kappaB-decoy oligodeoxynucleotides (rcB-decoy) inhibiting the activity of NF-kappaB in the PC12 cells.</p><p><b>METHODS</b>PC12 cells cultivating in the 6 wells plate were divided into 3 groups, experimental group adding kappaB-decoy complex (6 microg DNA/well), the control group adding scrambled-decoy complex, the normal group adding lipid-Lipofectamine 2000, transfer and cultivate 48 h, then lipopolysaccharide (LPS, 200 ng/ml) was added in the cells for 0.5-4 h. The immunocytochemistry and Western blot were used to measure the expression or the activity of NF-kappaB in PC12 cells.</p><p><b>RESULTS</b>In PC12 cells, compared with normal group, the expression of NF-kappaB enhanced obviously with the time of the stimulation of LPS in scrambled-decoy treated control group (P < 0.01), in 2-4 h the level reached the peak; the expression of NF-kappaB showed the stable level with the time of the stimulation of LPS in kappaB-decoy treated experimental group, compared with the control group, the expression levels were obviously lower than the respective time point of control groups (P < 0.01).</p><p><b>CONCLUSION</b>kappaB-decoy could reduce the expression of NF-kappaB in the normal PC12 cells and inhibit the activity of NF-cB in the pathologic PC12 cells.</p>