Mechanism of apoptotic effects induced by 5-fluorouracil on human liver carcinoma Bel7402 cell line / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 968-971, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-340407
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of endogenous nitric oxide (NO) on the ability of 5-fluourouracil (5-FU) to induce apoptosis in the liver carcinoma Bel7402 cell line, and to observe the anti-tumor mechanism and effective adjuvant of 5-FU.</p><p><b>METHODS</b>Cells were cultured under routine conditions with Dulbecco's modified Eagle's medium (DMEM) without L-Arginine (L-Arg). We observed the expression of inducible nitric oxide synthase (iNOS) and apoptosis of cells induced by 5-FU with L-Arg added to the medium. The production of nitric oxide was determined by the cell expression of iNOS detected by immunohistochemical staining, and by the concentrations of nitrite and nitrate in the supernatant.</p><p><b>RESULTS</b>5-fluourouracil significantly increased the iNOS expression to 0.1687 +/- 0.01968 (P < 0.05, vs control group), and the concentration of nitric oxide to 213 +/- 30.2 micromol/L (P < 0.05, vs control group). The apoptotic cell rate increased significantly to 17.85 +/- 0.78%, while the necrotic cell rate decreased to 3 2.99 +/- 0.83% (P < 0.05,compared with the 5-FU group). N(omega)-nitro-L-Arginine methyl ester (L-NAME), the antagonist of L-Arg, can block the apoptotic effects of endogenous nitric oxide.</p><p><b>CONCLUSIONS</b>5-FU had a synergistic effects with L-Arg by increasing the production of endogenous nitric oxide. Endogenous nitric oxide plays an important role in the process where 5-FU induces apoptosis in liver carcinoma cells. L-Arg may be a good adjuvant for chemotherapy with 5-FU.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Arginina
/
Inmunohistoquímica
/
Células Tumorales Cultivadas
/
Apoptosis
/
Óxido Nítrico Sintasa
/
Óxido Nítrico Sintasa de Tipo II
/
Fluorouracilo
/
Neoplasias Hepáticas
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2002
Tipo del documento:
Artículo
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