Effects of rhG-CSF Stimulation in vitro on the Adhesion and Polarization of Human CD4⁺T Lymphocytes / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1702-1706, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-340433
ABSTRACT
The adhesion and polarization of T lymphocytes involved in the adhesive interaction of lymphocyte function-associated antigen 1 (LFA-1) with its ligand intercellular adhesion molecule 1 (ICAM-1). This study was aimed to investigate the effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) stimulation in vitro on the adhesion and polarization of CD4⁺ T cells of healthy human in peripheral blood. The peripheral blood mononuclear cells were collected from 12 healthy volunteers. The CD4⁺ T cells were sorted by miniMACS. The sorted CD4⁺ T cells were incubated with rhG-CSF for 24 h, then the adhesion and polarization of CD4⁺ T cells activated by stroma cell-derived factor -1α (SDF-1α) and ICAM-1 were detected by ELISA and inverted microscope. The results showed that the percentage of adhesion CD4⁺T cells in the experimental group (rhG-CSF acting on the healthy adult volunteers) (61.9 ± 5.9)% was lower than that in the control group (healthy adult volunteers without rhG-CSF stimulation) (68.3 ± 7.3)% (P < 0.05). The percentage of polarized CD4⁺T cells in the experimental group (24.3 ± 4.3)% was also lower than that in control group (47.1 ± 5.1)% (P < 0.05). It is concluded that the adhesion and polarization of CD4⁺T lymphocytes can be inhibited after rhG-CSF stimulation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Técnicas In Vitro
/
Proteínas Recombinantes
/
Leucocitos Mononucleares
/
Activación de Linfocitos
/
Linfocitos T CD4-Positivos
/
Adhesión Celular
/
Movimiento Celular
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Factor Estimulante de Colonias de Granulocitos
/
Antígeno-1 Asociado a Función de Linfocito
Límite:
Anciano
/
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2014
Tipo del documento:
Artículo
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