Platelet-derived growth factor-BB inhibited p21(WAF1) expression partially through transforming growth factor-beta signalling system in vascular smooth muscle cell / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 160-165, 2010.
Article
en Zh
| WPRIM
| ID: wpr-341262
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To assess if the modulating effect of platelet-derived growth factor (PDGF)-BB on p21(WAF1) was mediated by upregulating transforming growth factor (TGF)-beta(1) expression in vascular smooth muscle cells (VSMC).</p><p><b>METHODS</b>TGF-beta(1) mRNA and protein expressions were measured by reverse transcription-PCR and ELISA, the protein expressions of p21(WAF1) and the downstream TGF-beta signalling including TGF-beta type I receptor (ALK-5 in VSMC), Smurf2, pSmad2/3, Smad4, Smad7 were detected by Western blot.</p><p><b>RESULTS</b>PDGF-BB significantly upregulated the expressions of TGF-beta(1) at mRNA (0.79-fold) and protein (1.98-fold) levels in VSMC, significantly inhibited the expression of p21(WAF1) (-67 +/- 12)%, and enhanced the expressions of ALK-5, pSmad2/3, Smad4, Smurf2 protein by 1.21-fold, 0.95-fold, 0.69-fold and 2.55-fold respectively, inhibited Smad7 expression (-65 +/- 9)%, these alterations were partially restored by anti-TGF-beta(1) neutralizing antibody.</p><p><b>CONCLUSIONS</b>These findings suggested that PDGF-BB inhibited p21(WAF1) expression in VSMC partially through upregulating TGF-beta(1) expression via PDGF-BB and TGF-beta signalling pathways.</p>
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Índice:
WPRIM
Asunto principal:
Farmacología
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Factor de Crecimiento Derivado de Plaquetas
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Transducción de Señal
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División Celular
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Células
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Ratas Sprague-Dawley
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Proteínas Proto-Oncogénicas c-sis
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Biología Celular
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Miocitos del Músculo Liso
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Inhibidor p21 de las Quinasas Dependientes de la Ciclina
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Año:
2010
Tipo del documento:
Article