The protection mechanisms of glycine against liver injury induced by lipopolysaccharides / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 574-577, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-341297
ABSTRACT
<p><b>OBJECTIVE</b>To explore the protective mechanisms of glycine (Gly) on lipopolysaccharides (LPS) induced liver injury.</p><p><b>METHODS</b>BABL/c mice were randomly divided into a LPS group, in which the animals were intraperitoneally injected with 10 mg/kg LPS, and a Gly group, in which the mice were pretreated with a 5% Gly-containing diet for 3 days before receiving the same dose of LPS. The livers of the mice were examined for histopathological changes. The TNF alpha and interleukin-10 (IL-10) levels in the blood plasma were measured using ELISA analysis. The mRNA expression of TNF alpha, IL-10 and Toll-like receptor 4 (TLR4) in hepatic tissues were detected using RT-PCR analysis. Protein expression of TLR4 in livers was detected using immunohistochemistry.</p><p><b>RESULTS</b>The Gly group mice had an improved survival rate and attenuated LPS-induced pathological changes in the liver tissues in comparison with those of the LPS group animals. The TNF alpha levels [(1,852.80+/-126.64) pg/ml vs (708.83+/-51.29) pg/ml, P<0.05] in plasma, as well as the expression of TNF alpha (A 1.59+/-0.14 vs. 0.91+/-0.11, P<0.05) and TLR4 (A 0.97+/-0.12 vs. 0.53+/-0.11, P<0.05) mRNA in liver tissues were decreased. However, the levels of plasma interleukin-10 [(344.09+/-31.70) pg/ml vs (418.64+/-38.86) pg/ml, P<0.05] were significantly increased and the peaking time left, shifted.</p><p><b>CONCLUSIONS</b>Gly pretreatment could attenuate LPS -induced liver injury in mice, which may be associated with its role in down-regulating TLR4 expression and up-regulating IL-10 production.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Sangre
/
Regulación hacia Abajo
/
Regulación hacia Arriba
/
Lipopolisacáridos
/
Factor de Necrosis Tumoral alfa
/
Interleucina-10
/
Receptor Toll-Like 4
/
Glicina
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2006
Tipo del documento:
Artículo
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