Screening of proteins interacting with hepatitis C virus NS3 from T7-phage display library / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To screen and identify proteins that interact with hepatitis C virus NS3 by means of T7-phage display system.</p><p><b>METHODS</b>Hepatitis C virus NS3 was expressed by prokaryotic expression and used as a selected molecule to biopan the T7 select human liver cDNA library; the selected positive clones were identified using DNA sequencing and analyzed with BLAST program in GenBank.</p><p><b>RESULTS</b>After BLAST analysis in all the positive clones, the proteins which interacted with the hepatitis C virus NS3 were found to be serpin peptidase inhibitor, clade A, member 1 (SERPINA1) and cyclophilin-LC.</p><p><b>CONCLUSION</b>T7-phage display system is a convenient, rapid and effective method for screening interacting proteins. The proteins thus selected will provide an important means for studying the pathogenesis and carcinogenesis of HCV.</p>