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Coexpression of hepatitis B virus X gene and hepatitis C virus C gene in HepG2 cells and its effect on the expression of VEGF / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 529-531, 2006.
Artículo en Chino | WPRIM | ID: wpr-341317
ABSTRACT
<p><b>OBJECTIVE</b>To establish an experimental model of HCV C-HBV X co-expression protein and explore its effect on the expression of VEGF.</p><p><b>METHODS</b>The HBV X gene was recovered by enzyme excision and inserted into PBK-CMV and PBK-HCVC, and recombinant plasmids PBK-X and PBK-X-C were constructed. The plasmids PBK-CMV, PBK-X, PBK-HCVC and PBK-X-C were transfected into HepG2 cells with liposomes. After being selected by G418, resistant colonies were obtained. Reverse transcription PCR and Western blot were used to show HBV X and HCV core protein expression. VEGF was analyzed using immunohistochemical methods and Western blot.</p><p><b>RESULTS</b>The recombinant plasmid PBK-X-C expressed HBV X and HCV core protein efficiently under the control of the vectors promoter. VEGF and VEGF mRNA of the cells co-expressing HCV C-HBV X proteins were higher than those cells expressing HBV X, HCV C and vector alone.</p><p><b>CONCLUSION</b>HBV X-HCV C co-expression protein can increase the expression of VEGF of HepG2 cells. It suggests that HBV and HCV have a synergic action in the carcinogenesis.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Transfección / Expresión Génica / Regulación Viral de la Expresión Génica / Transactivadores / Proteínas del Núcleo Viral / Hepacivirus / Factor A de Crecimiento Endotelial Vascular / Células Hep G2 / Vectores Genéticos / Genética Tipo de estudio: Estudio pronóstico Límite: Humanos Idioma: Chino Revista: Chinese Journal of Hepatology Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Transfección / Expresión Génica / Regulación Viral de la Expresión Génica / Transactivadores / Proteínas del Núcleo Viral / Hepacivirus / Factor A de Crecimiento Endotelial Vascular / Células Hep G2 / Vectores Genéticos / Genética Tipo de estudio: Estudio pronóstico Límite: Humanos Idioma: Chino Revista: Chinese Journal of Hepatology Año: 2006 Tipo del documento: Artículo