Biological features and ultrastructure of human umbilical cord mesenchymal stem cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 382-386, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-341398
ABSTRACT
<p><b>OBJECTIVE</b>To isolate and culture human umbilical cord mesenchymal stem cells (MSCs) and explore their biological features and ultrastructure.</p><p><b>METHODS</b>After isolating MSCs from the human umbilical cord, the proliferation, cycle, and apoptosis were observed. The cell ultrastructure was observed under transmission electron microscope. The cytokines including vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin-like growth factor-1 (IGF-1) were detected using enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>Human umbilical cord MSCs had fibroblast-like morphology and increased proliferation capability. Ultrastructural analysis showed that the MSCs had active cellular metabolism and strong migration and differentiation capabilities. Meanwhile, they could secrete anti-apoptotic cytokines such as VEGF, IGF-1, and HGF.</p><p><b>CONCLUSION</b>Human umbilical cord MSCs can secrete many anti-apoptotic cytokine and have good biological features.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cordón Umbilical
/
Factor I del Crecimiento Similar a la Insulina
/
Ciclo Celular
/
Células Cultivadas
/
Factor de Crecimiento de Hepatocito
/
Apoptosis
/
Biología Celular
/
Factor A de Crecimiento Endotelial Vascular
/
Proliferación Celular
/
Células Madre Mesenquimatosas
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2011
Tipo del documento:
Artículo
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