Structural and functional analysis of the N-terminal region of death receptor 5 / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 33-38, 2011.
Article
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| ID: wpr-341463
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ABSTRACT
<p><b>OBJECTIVE</b>To investigate the structure and function of the N-terminal region (NTR) of death receptor 5 (DR5).</p><p><b>METHODS</b>A series of deletions of the DR5 extracellular domain (DR5-ECD) proteins were expressed in E.coli. and purified by affinity chromatography. The binding ability of these deletant proteins to AD5-10, a mouse anti-human DR5 monoclonal antibody, was evaluated by immunoblotting and ELISA.</p><p><b>RESULTS</b>Recombinant DR5-ECD proteins containing the NTR were recognized and bound by AD5-10, while the other deletant proteins without the NTR failed to interact with AD5-10.</p><p><b>CONCLUSION</b>There is an AD5-10 targeting site in the NTR of DR5, which may play a role in developing novel immunotherapies for cancers.</p>
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WPRIM
Asunto principal:
Unión Proteica
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Sitios de Unión
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Ingeniería Genética
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Química
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Eliminación de Gen
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Receptores del Ligando Inductor de Apoptosis Relacionado con TNF
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Vectores Genéticos
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Genética
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Metabolismo
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Anticuerpos Monoclonales
Límite:
Animals
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Humans
Idioma:
En
Revista:
Acta Academiae Medicinae Sinicae
Año:
2011
Tipo del documento:
Article