Construction of eukaryotic expression vector of EGFRi-IL-24 recombinant gene / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 395-399, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-341611
ABSTRACT
The epithelial growth factor receptor interference (EGFRi) was obtained by synthetic primers. Overlapping PCR was used to produce EGFRi-IL-24 fusion gene, which is linked by Gly4Ser3. After sequence analysis, EGFRi-IL-24 was cloned into expression vector pPIC9k; EGFRi-IL-24/pPIC9k was linearized with SacI,and then transformed to electroporated pastoris GS115. Subsequently, positive clone was selected by G418 and PCR, and its phenotype was determined by SDS-PAGE and MTT assay. The results demonstrated that EGFRi-IL-24 protein was expressed and shown to have the potential for use in researches of its biological function and in clinical application.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Pichia
/
Proteínas Recombinantes de Fusión
/
Interleucinas
/
Receptores ErbB
/
Vectores Genéticos
/
Genética
/
Metabolismo
/
Antineoplásicos
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2010
Tipo del documento:
Artículo
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