Isolation of endophytic fungi from Macleaya cordata and screening of sanguinarine-producing strains / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4288-4292, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-341867
ABSTRACT
Endophytic fungi were isolated from Macleaya cordata growing in Dabie Mountain by agar-block method, and then the endophytic fungi were grouped into different types based on their morphological characteristics, and thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) were employed to determine whether the metabolic substances contained sanguinarine or not, and then preliminarily identified by morphological method. The results showed that the leaves hosted the largest number of endophytes (96 isolates) followed by the stems (57 isolates) and finally the roots (28 isolates), respectively. Based on morphological characteristics the endophytic fungi were grouped into 26 types in our study. TLC and HPLC results showed that there was sanguinarine in the metabolic substances of BLH 51 strain. According to the morphological characteristic, the BLH 51 strain was identified as Fusarium proliferatum. All these indicated that the medicinal plant M. cordata harbors abundant endophytes, which could be a new source for the search of active secondary metabolites.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tallos de la Planta
/
Raíces de Plantas
/
Hojas de la Planta
/
Papaveraceae
/
Benzofenantridinas
/
Endófitos
/
Hongos
/
Isoquinolinas
/
Metabolismo
/
Microbiología
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2014
Tipo del documento:
Artículo
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