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Regulation of CD3, CD4 and CD8 expressions on PMA-activated human peripheral T cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences ; (6): 155-159, 2004.
Artículo en Chino | WPRIM | ID: wpr-341919
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of mitogen Phorbol 12-myristate 13-Acetate (PMA) on CD3, CD4 and CD8 expression of human T-lymphocytes.</p><p><b>METHODS</b>Peripheral blood mononuclear cells from 37 blood samples stimulated in vitro with PMA at different concentrations (2,5,10,20 and 50 ng/ml for 4 hours) and time (10 ng/ml for 2,4 and 6 hours) were analyzed by 4-color flow cytometry (FCM).</p><p><b>RESULTS</b>Under different PMA stimulation protocols,significant CD4 down-regulation was observed,which was negatively correlated with intracellular cytokine secretion (r= 0.601,P<.001), except for PMA stimulation at 10 ng/ml for 2-hours which showed no significant intracellular cytokine secretion. The expressions of CD3 and CD8 molecules after PMA activation were not significantly affected as compared with pre-activation. Among CD3 positive T lymphocytes, CD4/CD8 double-negative cells only account for 5.52%.</p><p><b>CONCLUSION</b>PMA has a significant down-regulation effect on CD4 molecules of Th cells, without altering the CD3 and CD8 expression. For quantitative analysis of Th1/Th2 variation, indirect method such as CD3(+)CD8( ) T cells can be used to define CD4(+) Th cells stimulated by PMA in the future.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Sangre / Acetato de Tetradecanoilforbol / Activación de Linfocitos / Linfocitos T / Antígenos CD4 / Química / Interleucina-4 / Interferón gamma / Antígenos CD8 Límite: Niño / Humanos Idioma: Chino Revista: Journal of Zhejiang University. Medical sciences Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Sangre / Acetato de Tetradecanoilforbol / Activación de Linfocitos / Linfocitos T / Antígenos CD4 / Química / Interleucina-4 / Interferón gamma / Antígenos CD8 Límite: Niño / Humanos Idioma: Chino Revista: Journal of Zhejiang University. Medical sciences Año: 2004 Tipo del documento: Artículo