Effect of fragile histidine triad gene on apoptosis of MGC-803 gastric cancer cells / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of fragile histidine triad (FHIT) gene on the apoptosis of gastric cancer cells.</p><p><b>METHODS</b>FHIT gene was transfected into gastric cancer cell line MGC-803 by liposome. Western blot and immunohistochemical assay were employed to detect the expression of FHIT. Apoptosis and cell cycle were analyzed by flow cytometry (FCM). Morphological changes were observed by light and electron microscope.</p><p><b>RESULTS</b>Stable expression of FHIT protein in transfected MGC-803 cells was obtained. The rates of G0/G1 phase and apoptosis cells were significantly higher in FHIT-transfected MGC-803 cells than those in control cells (64.4%compared with 49.5%and 32.0%compared with 12.2%, respectively).</p><p><b>CONCLUSION</b>The results indicate that FHIT gene might be involved in the apoptosis of gastric cancer cells.</p>