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Bioinformatics analysis to the differentially expressed genes of normal mucosa and carcinoma of colon / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences ; (6): 95-124, 2004.
Artículo en Chino | WPRIM | ID: wpr-341932
ABSTRACT
<p><b>OBJECTIVE</b>To systemically analyze the differentially expressed genes from normal mucosa and carcinoma of colon obtained by SSH method.</p><p><b>METHODS</b>An automatic platform for the analysis of nucleotides based on Linux was constructed, and one of the three subtracted libraries from SSH (T -N) was systematically analyzed by this platform. Part of the results was verified by semi- quantity RT-PCR.</p><p><b>RESULT</b>The automatic platform for the analysis of nucleotides based on Linux was successfully constructed. There were 15 contigs from the subtracted T-N libraries, among which 2 had no match with known genes in the GenBank. The expressions of genes Homo Sapiens thymosin beta 4 (THY) and Homo Sapiens HbxAg transactivated protein 2 (XTP) had trends of increase with the progress of normal tissue-adenoma-adenocarcinoma when verified by semi- quantity RT-PCR.</p><p><b>CONCLUSION</b>Linux-based automatic platform provides an efficient way to systematically analyze the nucleotide sequences, which may be used in study on the mechanism of colorectal carcinogenesis.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Colon / Neoplasias del Colon / Biología Computacional / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Perfilación de la Expresión Génica / Genética / Mucosa Intestinal / Metabolismo Límite: Femenino / Humanos / Masculino Idioma: Chino Revista: Journal of Zhejiang University. Medical sciences Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Colon / Neoplasias del Colon / Biología Computacional / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Perfilación de la Expresión Génica / Genética / Mucosa Intestinal / Metabolismo Límite: Femenino / Humanos / Masculino Idioma: Chino Revista: Journal of Zhejiang University. Medical sciences Año: 2004 Tipo del documento: Artículo