Determination of amlodipine in CYP3A4 cDNA-expressed cells by HPLC / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 510-513, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-341964
ABSTRACT
<p><b>OBJECTIVE</b>To establish a RP-HPLC method for the determination of amlodipine after metabolism by cytochrome P450 cDNA-expressed cells.</p><p><b>METHODS</b>The determination was performed on a C(18) reversed phase column with a mobile phase composed of acetonitrile phosphates buffer (4555, v/v, pH 4.5) with UV detection (lambda250nm). Propranolol was used as the internal standard.</p><p><b>RESULT</b>The standard curve was linear over the concentration range of 0.2 - 30.0 microg/ml (r=0.9993), and the limits of determination was 20 ng/ml (S/N >or=3), the limits of quantity was 0.2 microg/ml (recovery 104.0%, RSD 11.4%, n=5). The recovery for this assay was (98.2+/-2.4)%, precision for inter-assay and intra-assay was <10 % and 6 %, respectively.</p><p><b>CONCLUSION</b>The HPLC method established is simple, accurate and suitable for the determination of amlodipine in cytochrome p450 cDNA-expressed cells.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Bloqueadores de los Canales de Calcio
/
Transfección
/
Cromatografía Líquida de Alta Presión
/
Amlodipino
/
Sistema Enzimático del Citocromo P-450
/
Citocromo P-450 CYP3A
/
Genética
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2003
Tipo del documento:
Artículo
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