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Evaluation of Quality of Washed Platelets Stored in Platelet Additive Solutions / 대한수혈학회지
Article en Ko | WPRIM | ID: wpr-34206
Biblioteca responsable: WPRO
ABSTRACT
BACKGROUND: Because of a lack of substances for platelet (PLT) metabolism and preservation, normal saline (NS) washed PLTs can only be stored for short lengths of time. However, the use of platelet additive solutions (PAS) could help solve this problem. In this study, the in vitro quality of NS washed platelets (wPLTs) stored in two types of PAS were compared with those of wPLTs stored in NS. METHODS: Five units of NS washed apheresis platelets were pooled aseptically and separated into five aliquots for storage in NS only as well as T-PAS+ (Terumo BCT, Lakewood, CO, USA) and CompoSol PS (Fenwal, Lake Zurich, IL, USA) with or without 15 mM glucose. The parameters of wPLTs quality were assessed up to 48 hrs after washing and the whole experiment was repeated 10 times independently. RESULTS: wPLTs in two kinds of PAS had better quality than wPLTs in NS, and wPLTs in T-PAS+ showed better quality than those in CompoSol PS. PAS-stored wPLTs with added glucose maintained stable CD62P and Annexin V expression during storage, but exhibited increased lactate accumulation. Evaluation of in vitro quality revealed that all wPLTs had a rating of 4 immediately after washing. However, only T-PAS+-stored wPLTs with glucose maintained a rating of 4 up to 48 hrs of post-washing. CONCLUSION: Using PAS storage for wPLTs may be beneficial compared to NS. The results presented herein suggest that T-PAS+ containing glucose has the potential to extend storage time by up to 48-hours.
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Texto completo: 1 Índice: WPRIM Asunto principal: Eliminación de Componentes Sanguíneos / Plaquetas / Conservación de la Sangre / Técnicas In Vitro / Lagos / Anexina A5 / Ácido Láctico / Glucosa / Metabolismo Idioma: Ko Revista: Korean Journal of Blood Transfusion Año: 2017 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Eliminación de Componentes Sanguíneos / Plaquetas / Conservación de la Sangre / Técnicas In Vitro / Lagos / Anexina A5 / Ácido Láctico / Glucosa / Metabolismo Idioma: Ko Revista: Korean Journal of Blood Transfusion Año: 2017 Tipo del documento: Article