Reprogramming of adult human neural stem cells into induced pluripotent stem cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1138-1143, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-342223
ABSTRACT
<p><b>BACKGROUND</b>Since an effective method for generating induced pluripotent stem cells (iPSCs) from human neural stem cells (hNSCs) can offer us a promising tool for studying brain diseases, here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors.</p><p><b>METHODS</b>NSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients. When combined with four factors (OCT3/4, SOX2, KLF4, and c-MYC), iPSCs colonies were successfully obtained.</p><p><b>RESULTS</b>Morphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties, which include the ability to differentiate into all three germ layers both in vitro and in vivo.</p><p><b>CONCLUSION</b>Our method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Inmunohistoquímica
/
Diferenciación Celular
/
Células Cultivadas
/
Proteínas Proto-Oncogénicas c-myc
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Biología Celular
/
Factores de Transcripción de Tipo Kruppel
/
Factor 3 de Transcripción de Unión a Octámeros
/
Reprogramación Celular
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2013
Tipo del documento:
Artículo
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