Mutation of lambdapL/pR-cI857 system for production of bacterial ghost in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1423-1430, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-342384
ABSTRACT
Bacterial ghost is intact envelope of Gram-negative bacteria, which is produced by the function of the lysis gene E from bacteriophage PhiX174. The expression of the lysis gene E is usually controlled by the thermosensitive lambdapL/pR-cI857 promoter. In this study, we described a mutation (T --> C) at the ninth nucleotide of the OR2 in the lambdapR promoter of the lambdapL/pR-cI857 system by overlap PCR. The bacteriolytic assay showed that the mutation in the lambdapL/pR-cI857 system enhanced the temperature of repressing the expression of gene E up to 37 degrees C. The lysis efficiency of altered lambdapR promoter in Escherichia coli DH5a and avian pathogenic E. coli DE17 was up to 99.9%. The expanded range of temperature will benefit for the production of bacterial ghost.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Bacteriólisis
/
Proteínas Virales
/
Virología
/
ADN Bacteriano
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Membrana Celular
/
Regulación de la Expresión Génica
/
Regiones Promotoras Genéticas
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2012
Tipo del documento:
Artículo
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