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Heat shock protein 27 enhances the inhibitory effect of influenza A virus NS1 on the expression of interferon-beta / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1205-1215, 2012.
Artículo en Chino | WPRIM | ID: wpr-342404
ABSTRACT
Heat shock protein 27 (HSP27) is a member of the small heat shock proteins (sHSP) and has multiple functions, it also plays an important role in the life cycle of some viruses. To investigate the regulatory effect of HSP27 during influenza virus infection, we cloned and expressed human HSP27 in both prokaryotic and eukaryotic cells, and demonstrated that HSP27 interacted with influenza A virus NS1 protein both in vivo and in vitro. Luciferase assay showed that HSP27 inhibited the expression of interferon-beta (IFN-beta) in infected cells, and independent of its phosphorylation. Moreover, HSP27 enhanced the inhibitory effect of NS1 on the expression of IFN-beta. Further analysis indicated that HSP27 exerted the inhibitory effect probably through influencing MDA5 of the RIG-I like helicase (RLH) pathway. The results suggested that HSP27 play a role in the innate immunity of infected cells, contributed to our understanding of the regulatory effect of host factors during influenza virus infection.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Regulación Viral de la Expresión Génica / Línea Celular / Interferón beta / Proteínas no Estructurales Virales / Alergia e Inmunología / Gripe Humana / Proteínas de Choque Térmico HSP27 / Células HEK293 / Genética Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2012 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Regulación Viral de la Expresión Génica / Línea Celular / Interferón beta / Proteínas no Estructurales Virales / Alergia e Inmunología / Gripe Humana / Proteínas de Choque Térmico HSP27 / Células HEK293 / Genética Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2012 Tipo del documento: Artículo