Enhanced cellulase production of Penicillium decumbens by knocking out CreB encoding a deubiquitination enzyme / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 959-972, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-342425
ABSTRACT
Penicillium decumbens T. is an important filamentous fungus for the production of cellulases to effectively degrade lignocellulose for second generation biofuel production. In order to enhance the capability of Penicillium decumbens to produce cellulases, we constructed a creB (a deubiquitinating enzyme encoding gene) deletion cassette, and generated a creB knockout strain with homologous double crossover recombination. This mutation resulted in a detectable decrease of carbon catabolite repression (CCR) effect. The filter paper activity, endoglucanase activity, xylanase activity and exoglucanase activity of the deltacreB strain increased by 1.8, 1.71, 2.06 and 2.04 fold, respectively, when comparing with the parent strain Ku-39. A 2.68 fold increase of extracellular protein concentration was also observed. These results suggest that the deletion of creB results in CCR derepression. These data also suggest that CREB influences cellulase production of Penicillium decumbens. In generation, this study provides information that can be helpful for constructing cellulase hyper-producing strain.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Penicillium
/
Endopeptidasas
/
Recombinación Genética
/
Celulasa
/
Proteínas Mutantes
/
Ubiquitinación
/
Técnicas de Inactivación de Genes
/
Proteínas Ubiquitinadas
/
Genética
/
Lignina
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2012
Tipo del documento:
Artículo
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