Single B cell monoclonal antibody technologies and applications / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 651-660, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-342453
ABSTRACT
Monoclonal antibodies (mAbs) contribute a lot to the development of numerous fields in life science as a pivotal tool in modern biological research. Development of the PCR methods and maturation of antibody production have made it possible to generate mAbs from single human B cells by single cell RT-PCR with successional cloning and expression in vitro. Compared to traditional monoclonal antibody technologies, single B cell technologies require relatively fewer cells, which are highly efficient in obtaining specific mAbs in a rapid way with preservation of the natural heavy and light chain pairing. With so many advantages, single B cell technologies have been proved to be an attractive approach for retrieval of naive and antigen-experienced antibody repertoires generated in vivo, design of rationale structure-based vaccine, evaluation and development of basic B cell biology concepts in health and autoimmunity, and prevention of infectious diseases by passive immunization and therapy for disorders. Accordingly, this review introduced recent progresses in the single B cell technologies for generating monoclonal antibodies and applications.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Linfocitos B
/
Técnicas Inmunológicas
/
Biología Celular
/
Alergia e Inmunología
/
Genética
/
Metabolismo
/
Anticuerpos Monoclonales
/
Especificidad de Anticuerpos
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2012
Tipo del documento:
Artículo
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