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Radiolabeling LyP-1 peptide and preliminary biodistribution evaluation in mice bearing MDA-MB-435 xenografts / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 471-475, 2013.
Artículo en Inglés | WPRIM | ID: wpr-342560
ABSTRACT
<p><b>BACKGROUND</b>Recent studies have shown the LyP-1 peptide can home to either tumor lymphatics or the tumor cells and be internalized by targeted cells. This study aimed to investigate the possibility of using Na(131)I labeled LyP-1 peptide as an imaging agent or a therapeutic radiopharmaceutical in breast carcinoma and its metastasis.</p><p><b>METHODS</b>The 10-mer cyclic peptide contained the LyP-1 sequence (YCGNKRTRGC) was synthesized by the solid phase method. Disulfide bonds between the cysteines maintain the cyclic structure. The LyP-1 peptide was labeled with Na(131)I using the chloramine-T method. The [(131)I] LyP-1 peptide and a [(131)I] control peptide were injected via tail vein into nude mice bearing MDA-MB-435 tumor xenografts. Biodistribution and imaging results in vivo were obtained.</p><p><b>RESULTS</b>The labeling efficiencies of LyP-1 peptide reached 80% ± 5% (n = 5). The radiochemical purity was about 96%. The radiochemical purity of the labeled compound remains 92% at 24 hours in human serum at 37°C. In the biodistribution studies, the [(131)I] LyP-1 peptide accumulated in the tumor to a higher level than in other organs. The [(131)I] LyP-1 peptide can successfully image the tumor in nude mice bearing MDA-MB-435 tumor xenografts.</p><p><b>CONCLUSIONS</b>The LyP-1 peptide could be effectively labeled with Na(131)I and the labeled compound is stable in human serum at 37°C for 24 hours. The high specificity of [(131)I] LyP-1 peptide suggests it may be a promising new radiotracer for identifying tumors.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Péptidos Cíclicos / Neoplasias de la Mama / Tomografía Computarizada de Emisión de Fotón Único / Química / Línea Celular Tumoral / Diagnóstico / Xenoinjertos / Ratones Desnudos Tipo de estudio: Estudio diagnóstico Límite: Animales / Femenino / Humanos Idioma: Inglés Revista: Chinese Medical Journal Año: 2013 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Péptidos Cíclicos / Neoplasias de la Mama / Tomografía Computarizada de Emisión de Fotón Único / Química / Línea Celular Tumoral / Diagnóstico / Xenoinjertos / Ratones Desnudos Tipo de estudio: Estudio diagnóstico Límite: Animales / Femenino / Humanos Idioma: Inglés Revista: Chinese Medical Journal Año: 2013 Tipo del documento: Artículo