The effect of fluid shear stress on the NO synthesis of rat osteoblast-like cells / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 575-578, 2004.
Article
en Zh
| WPRIM
| ID: wpr-342661
Biblioteca responsable:
WPRO
ABSTRACT
This study sought to elucidate the function of NO during the signal transduction wherein fluid shear stress regulates the proliferation and differentiation of osteoblast cells. The isolated rat osteoblast-like cells were exposed to fluid shear stress 12 dyn/cm2 for 5, 10, 15, 30, 60 and 120 min respectively with the use of a flow chamber. The NO release was examined. After the exposure to fluid shear stress, the NO synthesis of rat primary osteoblast-like cells increased significantly (P<0.05) when compared with the control. After 60 minutes of exposure, the release of NO began to increase significantly (P<0.05), but no significant increase as such was seen in the control (P>0.05). NO synthesis may be one of the signal transduction pathways which transduce the fluid shear stress into osteoblast cells. In early stage, it may be induced by cNOS and in late stage by iNOS.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Osteoblastos
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Estrés Mecánico
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Transducción de Señal
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Diferenciación Celular
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Células Cultivadas
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Óxido Nítrico Sintasa
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Biología Celular
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Resistencia al Corte
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Proliferación Celular
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Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2004
Tipo del documento:
Article