Freeze-drying of oxygen-carrying substances and storage stability of the freeze-dried solids / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 678-693, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-342766
ABSTRACT
Oxygen-carrying substances based on poly-placental hemoglobin were put into freeze-drying. Sucrose was chosen to inhibit the methmoglobin(MetHb) formation. MetHb content, ultravioletes(UV) spectrum, Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), High performance liquid chromatography (HPLC) were monitored during freeze-drying. As a result, when the mass ratio of sucrose to protein was above 0.5, MetHb formation was under control;the characteristic absorption of UV spectrum, SDS-PAGE and HPLC showed no visible change. Freeze-dried solids were kept under room temperature and refrigerator for 3 months. As a result, MetHb formation depended upon storage temperature and the mass ratio of sucrose to protein. For group C in which the mass ratio of sucrose to protein was 1.0; there is no marked change in MetHb content, UV spectrum, SDS-PAGE and HPLC after 3 months.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Placenta
/
Sacarosa
/
Hemoglobinas
/
Metahemoglobina
/
Química
/
Cromatografía Líquida de Alta Presión
/
Estabilidad de Medicamentos
/
Almacenaje de Medicamentos
/
Electroforesis en Gel de Poliacrilamida
Límite:
Femenino
/
Humanos
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2008
Tipo del documento:
Artículo
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