The study of apoptosis and mechanism of cells exposed to steep pulse / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 637-641, 2008.
Article
en Zh
| WPRIM
| ID: wpr-342774
Biblioteca responsable:
WPRO
ABSTRACT
This experiment was designed to study the apoptosis and related mechanism of adherent liver tumor cells (SMMC-7721) and adherent normal liver cells (HL-7702) when they were exposed to the steep pulse generated by the steep pulse apparatus for tumor treatment. The results showed that the steep pulse of 200 V could induce tumor cells apoptosis. The tumor cells presented with their apoptosis when they were exposed to the steep pulse from 200 V to 250 V. Laser scanning confocal microscopy was used to make a real time study of calcium burst when the adherent tumor cells were exposed to the steep pulse. The results showed:On the condition of no extracellular Ca2+, the concentration of Ca2+ in tumor cells exposed to the steep pulse of 150 V did not change; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 200 V decreased; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 250 V decreased more evidently. On the condition of existing extracellular Ca2+, the concentration of Ca2+ in tumor cells exposed to the steep pulse of 150 V did not change; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 200 V decreased little; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 250 V reduced little, too. Maybe the change of calcium burst in the tumor cells is the mechanism of apoptosis when cells are exposed to the steep pulse.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Patología
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Efectos de la Radiación
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Células Tumorales Cultivadas
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Calcio
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Apoptosis
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Microscopía Confocal
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Hepatocitos
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Biología Celular
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Electricidad
/
Campos Electromagnéticos
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2008
Tipo del documento:
Article