Construction and characterization of a novel somatostatin prokaryotic expression / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 995-998, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-342804
ABSTRACT
In the current work, the fusion gene including somatostatin (SS) and the hepatitis B surface antigen gene was cloned into a balanced lethal system plasmid (pYA3493), and then transformed into asd- attenuated Salmonella choleraesuis C500 strain, the positive transformant without antibiotic resistance gene was confirmed by restriction analysis and DNA sequencing, designated as pYA-SS. The expression and immunogenicity of fusion protein were detected by SDS-PAGE and Western blot analysis. These results show that the recombinant prokaryotic expression plasmid pYA-SS could express the SS fusion protein with good immunogenicity in C500 strain. In above all, this study could provide reliable materials to develop novel, good and safe vaccine in enhancing the growth of animals.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
Células Procariotas
/
Salmonella arizonae
/
Proteínas Recombinantes de Fusión
/
Somatostatina
/
Clonación Molecular
/
Fusión Artificial Génica
/
Alergia e Inmunología
/
Genética
/
Antígenos de Superficie de la Hepatitis B
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2008
Tipo del documento:
Artículo
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