Screening of T7 phage-display cDNA library from liver of Microtus fortis with extracts of schistosomulum and characterization of the positive clones / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 733-739, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-342843
ABSTRACT
Microtus fortis is naturally resisitent to Schistosoma japonicum. In order to find schistosome-resistence-related genes of Microtus fortis, a T7 phage-display cDNA library from liver of Microtus fortis was screened with the soluble lysate of schistosomula. The specific phages were enriched 375-fold after 3 rounds of biopanning. Ninety-two positive clones picked at random were sequenced and 19 ESTs including 6 unreported genes were obtained. Compared with the negative phage clone control, five positive clones, No.4 (GenBank Accession No. EW968294), No.13 (GenBank Accession No. EW968303), No.14 (GenBank Accession No. EW968304), No.15 (GenBank Accession No. EW968305) and No.18 (GenBank Accession No. EW968308) could induce significantly higher schistosomula mortality rate when co-cultivated with schistosomula. According to the function analysis and the shistosomula-killing effect in vitro, the genes encoding CASP8 and FADD-like apoptosis regulator isoform protein, alpha-2-HS-glycoprotein, M4 protein, R3H domain (binds single-stranded nucleic acids) isoform 2 and 3 previously unreported proteins (No.14, No.15 and No.18) obtained here, were schistosomiasis-resistence-related genes of Microtus fortis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Parasitología
/
Schistosoma japonicum
/
Biblioteca de Genes
/
Química
/
Arvicolinae
/
Clonación Molecular
/
Genes de Helminto
/
Bacteriófago T7
/
Etiquetas de Secuencia Expresada
/
Genética
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2008
Tipo del documento:
Artículo
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