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Promoter activity of different promoters in recombinant baculovirus-infected Sf9 cells / 生物工程学报
Chinese Journal of Biotechnology ; (12): 598-603, 2008.
Artículo en Chino | WPRIM | ID: wpr-342864
ABSTRACT
To compare the activity of different promoter in baculovirus-insect system, a series of recombinant baculoviruses were generated harboring the E-GFP reporter gene under the control of one of 5 promoters, including the ie1 promoter of shrimp white spot syndrome virus (WSSV), the truncated ie1 (mie1) promoter, the ETL promoter of the baculovirus, the elongated ETL (mETL) promoter, and the polyhedron promoter (P(PH)) of the baculovirus. The expression efficiency of the E-GFP reporter gene in the recombinant baculovirus-infected Sf9 cells was determined by flow cytometry. The results showed that both ie1 and mETL promoters had a strong promoter activity at early phase, while P(PH) showed a strong promoter activity at late phase. The ie1 promoter suggested the strongest promoter activity. The homologous region 1 (hr1) was also found to enhance the ETL promoter activity.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Virología / Proteínas Recombinantes / Transfección / Baculoviridae / Regiones Promotoras Genéticas / Penaeidae / Virus del Síndrome de la Mancha Blanca 1 / Proteínas Fluorescentes Verdes / Genética / Insectos Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Virología / Proteínas Recombinantes / Transfección / Baculoviridae / Regiones Promotoras Genéticas / Penaeidae / Virus del Síndrome de la Mancha Blanca 1 / Proteínas Fluorescentes Verdes / Genética / Insectos Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2008 Tipo del documento: Artículo