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Heterologous genes expression on Escherichia coli chromosome lac operon using Red recombination / 生物工程学报
Chinese Journal of Biotechnology ; (12): 576-580, 2008.
Artículo en Chino | WPRIM | ID: wpr-342868
ABSTRACT
To achieve efficient and stable expression of heterologous exogenetic protein or antigen in E. coli chromosome, the luciferase report gene was knocked in lacZ site of chromosome lac operon by using Red recombination system and selection-counterselection kan/sacB technology. The quantitative analysis of exogenous gene expression indicated that the target gene could be efficiently expressed at lacZ site of lac operon. The results confirmed the efficient screening and stable expression of heterologous protein or antigen on chromosome by using the recombinant engineering technique. This study demonstrated that the chromosome could be used as a vector for heterologous protein or antigen and the stable expression of exogenous gene on E. coli chromosome had no side effect on the bacterial growth and propagation.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Recombinación Genética / Cromosomas Bacterianos / Clonación Molecular / Bacteriófago lambda / Genes Reporteros / Escherichia coli / Técnicas de Sustitución del Gen / Genética / Operón Lac / Luciferasas Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Recombinación Genética / Cromosomas Bacterianos / Clonación Molecular / Bacteriófago lambda / Genes Reporteros / Escherichia coli / Técnicas de Sustitución del Gen / Genética / Operón Lac / Luciferasas Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2008 Tipo del documento: Artículo