Determination of ofloxacin in human fallopian tube, uterus and serum by high performance liquid chromatography / 药学学报
Acta Pharmaceutica Sinica
;
(12): 134-137, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-343383
ABSTRACT
<p><b>AIM</b>To establish a method for determineation of the concentration of ofloxacin in human fallopian tube, uterus and serum.</p><p><b>METHODS</b>The separation was performed on a Spherisob C18 column (Hypersil, 250 mm x 4.6 mm ID, 5 microns) with a mobile phase of acetonitrile-0.01 moL.L-1 potassium dihydrogen phosphate-0.5 mol.L-1 tetrabutylammonium bromide (9914, pH 2.5). The flow rate was 1.0 mL.min-1 and detection was at 294 nm. The samples were homogenated or ground to powder after freezing with liquid nitrogen. 1% triton-100 and certain volume of ethylacetate-isopropanol (101) were added, shaken and centrifuged. Then the entire organic layer was transferred to a tube and vacuum dried. The residue was reconstituted in the mobile phase for HPLC.</p><p><b>RESULTS</b>There was a linear relationship between the peak area ratio and the ofloxacin concentration over the range of 0.2-8.0 micrograms.mL-1. The limits of detection was 40 ng.mL-1. Using this method to determine the ofloxacin concentrations in relevant organs as well as in the plasma of patients of the Department of Gynecology, and achieved satisfactary results.</p><p><b>CONCLUSION</b>The method can be applied to assay the ofloxacin concentration in human tissues. Ofloxacin was well distributed in woman fallopian tube, uterus and serum after single oral administration.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Útero
/
Sangre
/
Farmacocinética
/
Distribución Tisular
/
Ofloxacino
/
Cromatografía Líquida de Alta Presión
/
Trompas Uterinas
/
Metabolismo
/
Antiinfecciosos
Límite:
Adolescente
/
Adulto
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2002
Tipo del documento:
Artículo
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